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KMID : 0376219850220010015
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Chonnam Medical Journal
1985 Volume.22 No. 1 p.15 ~ p.23
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Oxidant-Induced Reduction in Red Cell Deformability as Related to Changes in the Membrane Spectrin of Erythrocytes
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李民化/Lee, Min-Wha
李起榮/梁誠烈/朴昞柱/尹士炫/Lee, Ki-Yung/Yang, Sung-Yeul/Park, Byung-Ju/Yoon, Sa-Hyun
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Abstract
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Effects of various oxidants such as peracetic acid (PAA), alloxan, quinone, and hydroquinone on the membrane spectra and cell deformability of rabbit erythrocytes were investigated to find out the possible relationships of reduced red cell deformability with changes in the spectra molecule.
Although the membrane lipid peroxidation occurred only in PAA-treated erythrocytes, quinone and hydroquinone as well as PAA-treated red cells revealed a decrease in the spectra components of the membrane proteins with a concomitant increase in high molecular weight protein complexes larger than spectra in molecular weight. The red cell deformability as assayed by erythrocyte filterability was also reduced by PAA, quinone and hydroquinone in the decreasing order. Alloxan was almost without effect on both the membrane spectra and cell deformability. Furthermore, the PAA-induced changes in spectra and reduction in cell deformability were partially reversed by the post-treatment of the erythrocytes with dithiothreitol. Thus, the magnitude of the spectra changes was well correlated to the reduction in cell deformability.
It is suggested from these results that the spectra damage in the red cell membrane is one of the factors leading to the decrease in red cell deformability while the membrane lipid peroxidation need not be a factor in the reduction of cell deformability.
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